Efficient fluorescence labeling of a large RNA through oligonucleotide hybridization
نویسندگان
چکیده
منابع مشابه
Efficient fluorescence labeling of a large RNA through oligonucleotide hybridization.
We present an efficient method of introducing fluorophore labels at selected locations in a large RNA. The method is based on specific and highly efficient hybridization between a fluorophore-containing DNA oligonucleotide and a modular hairpin loop replacing a functionally unimportant hairpin loop in the RNA. We demonstrate its feasibility using a 255-nucleotide RNA derived from the catalytic ...
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ژورنال
عنوان ژورنال: RNA
سال: 2005
ISSN: 1355-8382
DOI: 10.1261/rna.7180305